Study on the pathogenicity of dental biofilm by molecular genetic technics

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462801
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Kanagawa Dental College
• Principal Investigator: Hamada Nobushiro 神奈川歯科大学, 大学院歯学研究科, 教授 (20247315)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: グラム陽性桿菌 / Actinomyces naeslundii / バイオフィルム / 歯周炎

Outline of Final Research Achievements

A. naeslundii peptidoglycan induces the production of inflammatory cytokines and activates osteoclasts in alveolar bone resorption. Expressions of IL-1β, IL-6, and TNF-α mRNA were apparent in BALB/c mouse peritoneal macrophages stimulated with A. naeslundii PGN. In experimental periodontitis, the oral challenge of A. naeslundii resulted in the induction of alveolar bone loss in rats. These results suggest that peptidoglycan of A. naeslundii is an important virulence factor in the development of periodontitis. We could not succeed in the mutation of A. naeslundii fimbriae, however titanium oxide photocatalytic properties contribute to the reduction of bacterial biofilms and aid in the prevention of periodontal diseases.

Free Research Field

口腔細菌