2016 Fiscal Year Final Research Report
Effect of CCN2 on the osteocyte function regulating the osteoclast formation, and its possibility as a novel drug of osteoporosis
Project/Area Number |
26462810
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Functional basic dentistry
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Research Institution | Okayama University |
Principal Investigator |
Nishida Takashi 岡山大学, 医歯薬学総合研究科, 准教授 (30322233)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Keywords | CCN2/CTGF / 骨細胞様細胞 / 破骨細胞形成 / タモキシフェン誘導性Ccn2欠損マウス / 3次元培養 |
Outline of Final Research Achievements |
It is well-known that osteocytes play a role in the regulation of bone remodeling. However, the effect of CCN2/CTGF on the bone remodeling in osteocytes is unknown. Therefore, the aim of this study is the investigation of osteoclastogenesis via osteocytes stimulated by CCN2. A mouse osteocytic cell line, MLO-Y4 embedded into collagen gel containing recombinant CCN2 protein, promoted the osteoclast differentiation of RAW264.7 cells inoculated on the collagen gel, and osteocyte-like cells isolated from Ccn2-deficient mice inhibited the differentiation of osteoclasts under the same condition. These findings suggest that osteoclastogenesis is modulated by osteocytes stimulated by CCN2.
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Free Research Field |
口腔生化学 細胞生物学
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