Effect of CCN2 on the osteocyte function regulating the osteoclast formation, and its possibility as a novel drug of osteoporosis

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462810
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Okayama University
• Principal Investigator: Nishida Takashi 岡山大学, 医歯薬学総合研究科, 准教授 (30322233)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: CCN2/CTGF / 骨細胞様細胞 / 破骨細胞形成 / タモキシフェン誘導性Ccn2欠損マウス / 3次元培養

Outline of Final Research Achievements

It is well-known that osteocytes play a role in the regulation of bone remodeling. However, the effect of CCN2/CTGF on the bone remodeling in osteocytes is unknown. Therefore, the aim of this study is the investigation of osteoclastogenesis via osteocytes stimulated by CCN2. A mouse osteocytic cell line, MLO-Y4 embedded into collagen gel containing recombinant CCN2 protein, promoted the osteoclast differentiation of RAW264.7 cells inoculated on the collagen gel, and osteocyte-like cells isolated from Ccn2-deficient mice inhibited the differentiation of osteoclasts under the same condition. These findings suggest that osteoclastogenesis is modulated by osteocytes stimulated by CCN2.

Free Research Field

口腔生化学　細胞生物学