2016 Fiscal Year Final Research Report
Bone marrow-derived fibrocytes and/or macrophages interact with oral cancer cells and stimulate the malignant progression of cancer cells
| Project/Area Number |
26462823
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
OHTSUKA Masato 東海大学, 医学部, 講師 (90372945)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 癌細胞 / ニッチ / マクロファージ / 免疫抑制 / 接着 / マクロファージコロニー刺激因子 / M2-マクロファージ |
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| Outline of Final Research Achievements |
Cell-cell interactions among the cells consisting cancer cell niche was investigated. Bone marrow-derived lineage-positive (Lin+) blood cells proliferated and differentiated into Immunosuppressive/anti-inflammatory macrophage (Mφ), M2-Mφs, by cooperation with the bone marrow-derived mesenchymal stem cells (MSCs) under hypoxic condition: MSCs not only promoted proliferation of undifferentiated M2-Mφs, pre-M2-Mφs, in the Lin+ fraction via a proliferative effect of the MSCs-secreted macrophage colony-stimulating factor, but also promoted M2-Mφ polarization of the pre-M2-Mφs through cell-to-cell contact with the pre-M2-Mφs. The inhibitory experiments using the neutralizing antibodies and the adhesion molecule inhibitors suggested that the cell-to-cell adhesion through LFA-1 on pre-M2-Mφs and ICAM-1 on MSCs was supposed to promoted the M2-Mφ polarization. Thus, in the cancer niche, the induced M2-Mφs seemed to implicate in the progressive transformation of the oral cancer cells.
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| Free Research Field |
口腔生化学
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