Immunopathology of outer membrane vesicles of periodontopathic bacteria

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462866
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: NAKAO Ryoma 国立感染症研究所, 細菌第一部, 主任研究官 (10370959)
• Co-Investigator(Renkei-kenkyūsha): SENPUKU Hidenobu 国立感染症研究所, 細菌第一部, 室長 (20250186) ; OHNISHI Makoto 国立感染症研究所, 細菌第一部, 部長 (10233214) ; TAKAHASHI Hideyuki 国立感染症研究所, 細菌第一部, 主任研究官 (60321866) ; OGATA Yorimasa 日本大学松戸, 歯学部, 教授 (90204065) ; HIGUCHI Hideo 東京大学, 理学部, 教授 (90165093)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 歯周病 / 免疫 / 外膜ヴェシクル

Outline of Final Research Achievements

Gram-negative bacteria produce and release spherical, microstructural bodies called outer membrane vesicles (OMVs) during growth. As with other Gram-negative bacteria, OMVs are naturally produced by Porphyromonas gingivalis (Pg). In the present study, we identified immunodominant antigens recognized in serum samples from mice intranasally immunized with PgOMV by a sub-immunoproteome approach with the combination of two-dimensional gel electrophoresis and mass spectrometry analysis. In addition, we discovered a novel approach for purification and selective capture of PgOMV. PgOMV was found to bind to magnetic beads coated with epoxy groups in a noncovalent, species-specific manner.

Free Research Field

細菌学、分子生物学