Application of deciduous teeth pulp cell as supply source for induced pluripotent stem (iPS) cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462971
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Nagasaki University
• Principal Investigator: KAMASAKI Yoko 長崎大学, 病院(歯学系), 講師 (30253678)
• Co-Investigator(Renkei-kenkyūsha): FUKUMOTO Satoshi 東北大学, 歯学研究科(研究院), 教授 (30264253)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 乳歯由来歯髄細胞 / iPS細胞 / 多能性幹細胞 / GSK-3阻害剤（BIO)

Outline of Final Research Achievements

The 27 dental pulp cells were isolated from human exfoliated deciduous teeth of 20 children, who were healthy or had some kind of systemic disease. Only 8 dental pulp cells from deciduous teeth were able to be cultivated continuously and cryopreserved. The proliferative potential of these cells were varied widely.
All of these cells expressed 15 kinds of protein markers for the human pluripotent stem cell including Oct3/4,Nanog, Sox2, and so on. The kind of tooth and passage culture did not effect on the expression of all protein markers for the human pluripotent stem cell significantly. As the method for reprogramming of cells, application of Glycogen synthase kinase-3 (GSK-3) inhibitors (BIO) with low concentration (1μM) suppressed cell proliferation potential and increased expression level of Snail in 15 kinds of protein markers for the human pluripotent stem cell.

Free Research Field

小児歯科学