2016 Fiscal Year Final Research Report
Basic research toward the development of periodontal tissue regeneration implant using dentin sialophosphoprotein.
| Project/Area Number |
26462982
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Tsurumi University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 歯 / タンパク質 / 生理活性物質 / 再生 / 遺伝子 / 歯髄 / 象牙芽細胞 / 象牙質 |
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| Outline of Final Research Achievements |
We purified TGF-β-unbound or -bound DPP and DSP. The TGF-β isoform bound to DPP and DSP was identified as being TGF-β1 by both ELISA and LC-MS/MS analysis. DPP and DSP rescued the loss of TGF-β1 activity suggesting that they help retain TGF-β1 activity in porcine dentin.
We further fractionated DSPP-derived proteins from the dental pulp of developing porcine incisors. DSP was identified, but little DPP could be detected. Expression of the full-length Dspp mRNA by quantitative real-time polymerase chain reaction analysis was significantly higher in odontoblasts than in pulp, while expression of the DSP-only mRNA was almost equal in odontoblasts and in the pulp. Expression of the full-length Dspp mRNA was also significantly higher than the expression of DSP-only mRNA in odontoblasts. We conclude that the alternative polyadenylation of specific mRNAs of DSPP is regulated differently in pulp and differentiated odontoblasts.
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| Free Research Field |
歯科医用工学・再生歯学
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