2016 Fiscal Year Final Research Report
High order functionalization using the low dimensional nano space of titania nanotubes and application to DDS
| Project/Area Number |
26462988
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Osaka Dental University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 酸化チタン / チタニアナノチューブ / PDT / DDS |
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| Outline of Final Research Achievements |
Since one of the potential applications of titanium oxide nano-tubes (TNT) is to photodynamic therapy (PDT), the effects of reactive oxygen species (ROS) formed by TNT irradiated with ultraviolet (UV) rays were evaluated in HeLa cells.
An oxidative stress fluorescence probe was then used to visualize oxidative stress in HeLa cells, and revealed that the ROS formed by TNT led to oxidative stress in HeLa cells. A flow cytometric analysis was performed to characterize the cell status and distinguish between viable and unviable cells. Polyethylene glycol (PEG) modifications to the surfaces of TNT effectively facilitated adherence to cells, and the UV irradiation of HeLa cells during their incubation in culture medium containing PEG-modified TNT induced oxidative stress, thereby suppressing DNA repair.
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| Free Research Field |
ナノ材料科学
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