Development of novel cartilage regeneration method utilizing regenerative precursor cells and in vivo maturation promoting factor

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462996
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Gunma University (2016); The University of Tokyo (2014-2015)
• Principal Investigator: KANNO Yuki 群馬大学, 医学部附属病院, 助教 (80451813)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 再生前駆細胞 / 成熟促進因子 / 再生軟骨

Outline of Final Research Achievements

In this study, we identify regenerative precursor cells that constitute regenerative tissues in vivo, select them, clarify the cartilage maturation promoting factor in vivo, realize maturation before transplantation of cartilage regeneration tissue by utilizing both, It aimed to dramatically improve the characteristics of regenerated tissue. A PP plate seeded with cultured mouse chondrocytes for confirmation of the maturation promoting factor from the transplanting mother bed was transplanted into the abdominal cavity of a nude mouse. Ascites supernatant collected after transplantation and ascites cells were co-cultured with human chondrocytes and gene expression was evaluated. In the ascitic cell mRNA, the expression change was searched and the gene strongly inducing cartilage maturation was examined. For proteins in the ascites supernatant, analysis was performed to examine factors that promote cartilage maturation.

Free Research Field

顎口腔外科