Evaluation of animal-free systems in human deatal pulp cells using a spinal cord injury model

2016 Fiscal Year Final Research Report

• Project/Area Number: 26463001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Gifu University
• Principal Investigator: Kawaguchi Tomoko (武田知子) 岐阜大学, 医学部附属病院, 医員 (30509815)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 再生歯学

Outline of Final Research Achievements

Human dental pulp cells (hDPC) are a promising resource for regenerative medicine and tissue engineering. However, current protocols use reagents of animal origin (mainly fetal bovine serum) that carry the potential risk of infectious diseases and unwanted immunogenicity. Here, we report a chemically defined protocol to isolate and maintain the growth and differentiation potential of hDPCs. The chemically defined culture conditions can be used for the safe establishment of iPSCs and will find utility in applications for cell-based regenerative medicine.
The central nervous system in adult mammals cannot be repaired spontaneously after spinal cord injury (SCI). However, SCI treatment has improved in recent years following the development of cell transplantation therapy. We recently found that basic fibroblast growth factor (FGF2)-pre-treated hDPCs cultured under chemically defined conditions can improve recovery in rat SCI models.

Free Research Field

医歯薬学