Study of bone regeneration using osteoblast differentiation-inducing dental pulp-derived cell sheet

2016 Fiscal Year Final Research Report

• Project/Area Number: 26463021
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Yamamoto Toshiro 京都府立医科大学, 医学(系)研究科(研究院), 講師 (40347472)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 培養シート / 歯髄由来細胞 / 羊膜 / 骨分化誘導

Outline of Final Research Achievements

In the present study, we developed an osteoblast differentiation-inducing dental pulp-derived cell sheet on a human amniotic membrane by culturing the cell-membrane complex in a medium supplemented with osteoblast-inducer reagent. Implantation of the osteoblast differentiation-inducing dental pulp-derived cell sheet on the dorsal skin of nude mice led to the formation of calcified tissue. Similarly, implantation of the osteoblast differentiation-inducing sheet on periodontal bone defects in rats led to a significant increase in bone formation compared with the control sheet of cells cultured in a control medium.
These findings suggest that the osteoblast differentiation-inducing dental pulp-derived cell sheet on a human amniotic membrane may be useful for the regeneration of periodontal tissue.

Free Research Field

再生医療