Transportation research of dedifferentiated fat cells derived from the human buccal fat pad for maxillofacial cartilage regeneration

2017 Fiscal Year Final Research Report

• Project/Area Number: 26463033
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Osaka Dental University
• Principal Investigator: KUBO Hirohito 大阪歯科大学, 歯学部, 講師 (70388362)
• Co-Investigator(Kenkyū-buntansha): 橋本 典也 大阪歯科大学, 歯学部, 准教授 (20228430)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 脱分化脂肪細胞

Outline of Final Research Achievements

Human dedifferentiated fat (hDFAT) cells are thought to be a promising cell source for cartilage regeneration therapy. Nevertheless, the responses of hDFAT cells to bone morphogenetic proteins (BMPs) are still unclear. Recombinant BMP-4 (100 ng/mL) increased the expression of SOX9, SOX6, and aggrecan mRNAs in monolayer cells compared with that in cells treated with BMP-2 or BMP-7 on day 3. Chondrogenically differentiated hDFAT cells induced by CM containing BMP-4 showed higher expression of eight genes in monolayer cultures and nine genes in pellet cultures compared with those in control medium on day 14. Dorsomorphin attenuated the effects of BMP-4. These results showed that BMP-4 had the potential to modulate the early chondrogenesis of hDFAT cells under both monolayer and pellet cell culture conditions.

Free Research Field

口腔外科学