2016 Fiscal Year Final Research Report
Identification of cancer stem cell niche in oral cancer and their therapeutic application
| Project/Area Number |
26463035
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | がん幹細胞 / 腫瘍血管 |
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| Outline of Final Research Achievements |
In this study, we investigated the role of tumor endothelial cells (TECs) for maintenance of cancer cell stemness. We analyzed the crosstalk of cancer stem cells (CSCs) and endothelial cells in vitro. Treatment of cultured conditioned medium of CSC-enriched cells increased the expression of TEC markers in endothelial cells. We next analyzed the signal pathways after co-culture of cancer cells with TECs. NF-kB and ERK1/2 pathways were activated in cancer cells after co-culture with high grade tumor derived TECs. In addition, colony formation was observed by co-culture with cancer cells and TECs. These data suggested that TEC-expressing molecules are involved in maintenance of stemness in cancer cells.
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| Free Research Field |
医歯薬学
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