2016 Fiscal Year Final Research Report
The isolation of stem cells from the human exfoliated deciduous teeth by genetic engineering
| Project/Area Number |
26463109
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Orthodontics/Pediatric dentistry
|
|---|
| Research Institution | Niigata University |
|---|
Principal Investigator |
SAWAMI Tadashi 新潟大学, 医歯学総合研究科, 特任助教 (90710442)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | 再生歯学 / 神経幹細胞 |
|---|
| Outline of Final Research Achievements |
It was considered that to regenerate nerve cells is difficult, but the regeneration of nerve cells from neural stem cells gradually have been showed by the development of a recent study about regenerative medicine.Our aims in this study were to establish the way to isolate stem cells from the human exfoliated deciduous teeth by genetic engineering, and to prove the isolated stem cell effective as of material to tooth regeneration.
We showed that piggy Bac genetic electroporation using Neon was effective to the pulp cell of deciduous teeth and iPS cell. We also isolated dental stem cells from the human exfoliated deciduous teeth by genetic engineering, and showed a neural stem cell marker from the isolated stem cells. The nerve cell was admitted by in vitro differentiation derived from them.
|
| Free Research Field |
歯学
|
