2016 Fiscal Year Final Research Report
Involvement of epigenetic regulatory factors in respiration and swallowing during the suckling period
| Project/Area Number |
26463120
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
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| Research Collaborator |
TAKEI Hiroki
ISHIYAMA Misa
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | Mecp2 / Gad1 / 延髄呼吸中枢 / ヒストンアセチル化 / バルプロ酸 / エピジェネティクス |
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| Outline of Final Research Achievements |
Epigenetic effects of valproate (VPA) on Gad1 expression in the medullary ventral respiratory group (VRG) of neonatal Mecp2-deficient mice were examined. We found that the Gad1 mRNA level in the VRG of Mecp2-deficient mice was lower than that of wild-type mice while VPA treatment for 7 days increased Gad1 mRNA expression in the VRG of Mecp2-deficient mice on postnatal day 15. Acetylation levels of H3K9 and H4K5, the lysine residues of core histones, were increased with the VPA treatment in the Mecp2-deficient mice. These results suggest that the effects of VPA on Gad1 mRNA expression in the VRG of Mecp2-deficient mice are caused by the increase in the acetylation levels of core histones which may enhance Gad1 promoter activity.
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| Free Research Field |
小児歯科学
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