2016 Fiscal Year Final Research Report
Induction of Migration of Periodontal Ligament Cells by Selective Regulation of Integrin Expression
| Project/Area Number |
26463134
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Periodontology
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| Research Institution | Okayama University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | インテグリン / 細胞遊走 / 歯根膜 / 歯周組織 |
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| Outline of Final Research Achievements |
Periodontal ligament cells (PDLCs) are multipotent cells that can differentiate into osteogenic cells. It is necessary to induce migration of PDLCs for regeneration and homeostasis of periodontal tissue. Cell migration is regulated by local microenvironment, defined by coordination between soluble factors and extracellular matrix (ECM). Integrin-mediated cell adhesion to ECM provides essential signals for cell migration. To determine adhesion molecules responsible for migration of PDLCs, we examined expression profiles of integrin and ECM, and the integrin isoform-specific regulation of migration of PDLCs. The array analysis indicated that mRNA of integrin α2, α3, α4, and α5 were increased in migrating PDLCs. Anti-integrin α3 antibody and blocking peptides significantly increased migration of PDLCs, conversely anti-integrin α5 antibody decreased. Therefore, specific inhibition of integrin α3 may be useful to induce migration of PDLCs during regeneration of periodontal tissue.
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| Free Research Field |
歯周病学，歯周治療系歯学，歯周再生医学
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