2016 Fiscal Year Final Research Report
The roles of CREBH in non-alcoholic fatty liver
| Project/Area Number |
26500001
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Integrated Nutrition Science
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 非アルコール性脂肪肝 / 脂質代謝 |
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| Outline of Final Research Achievements |
In this study, we examined how CREBH is involved in lifestyle-related diseases, especially on the development and progression of nonalcoholic fatty liver. CREBH liver-specific KO (LKO) mouse using CRISPR/Cas9 system was successfully produced. The mouse exhibited a remarkable increase in blood lipids upon fasting. One of the causes is the increase of the transcription factor SREBPs which govern the expression of fatty acid and cholesterol synthesis genes. When LKO mice were fed with methionine choline deficient diet, which is a nonalcoholic fatty liver model, severe liver injury appeared in the early period. In this study, we have established a method for preparing new genetically modified mice, and clarified that deficiency of CREBH induces nonalcoholic fatty liver.
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| Free Research Field |
応用健康科学
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