2016 Fiscal Year Final Research Report
Quantitative phosphoproteomic analysis of mTOR signaling perturbation in glioblastoma stem cells
| Project/Area Number |
26505001
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
オミクス計測科学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | プロテオミクス / 膠芽腫幹細胞 / mTORシグナル / リン酸化 / SILAC |
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| Outline of Final Research Achievements |
Glioblastoma is the most malignant brain tumor with poor prognosis and shows high resistance to the conventional treatment such as chemotherapy or radiation. In this study, we performed global quantitative proteome and phosphoproteome analyses of EGF-dependent mTOR signaling in patient-derived gliblastoma stem cells through perturbation by mTOR specific inhibitor, Torin1.
As a result of integrated statistical and mathematical analyses of these large-scale data, it was strongly suggested that DNA damage checkpoint might be involved in the maintenance of stemness properties in the glioblastoma stem cells.
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| Free Research Field |
プロテオミクス
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