Characterization of gas- and solution-phase structures of not crystallizable proteins

2017 Fiscal Year Final Research Report

• Project/Area Number: 26505009
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: オミクス計測科学
• Research Institution: Yokohama City University
• Principal Investigator: AKASHI Satoko 横浜市立大学, 生命医科学研究科, 准教授 (10280728)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 蛋白質 / 生体分子 / 分析科学 / 質量分析

Outline of Final Research Achievements

The gas-phase and solution structures of not crystallizable proteins with intrinsically disordered regions were characterized by ion mobility mass spectrometry (IM-MS) in combination with small angle X-ray scattering (SAXS) analysis and molecular dynamics simulation (MD simulation). To analyze the structures　observed　in　the　ESI　mass　spectra,　computational　calculations　were performed for the canonical　nucleosome　core particle. To characterize the structure of nucleosome core particle with CG-rich DNA sequence, sample preparation was examined with 147 bp-DNA fragment.

Free Research Field

構造生物化学