The epigenetic regulation for DNA damage memory

2015 Fiscal Year Final Research Report

• Project/Area Number: 26550028
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Kyoto University
• Principal Investigator: Ikura Masae 京都大学, 放射線生物研究センター, 研究員 (40535275)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: DNA損傷記憶 / ヒストン化学修飾 / クロマチン構造変換 / クロマチン免疫沈降

Outline of Final Research Achievements

It is known that histone modifications including the phosphorylatin, acetylation, ubiquitination and methylation have been detected after induction of DNA damage. However, it is unclear whether these modifications are removed or still observed after DNA damage-repair. Thus we investigated the status of these modifications including the phosphorylated H2AX at the DNA damage-repaired sites by the chromatin immunoprecipitation (Chromation IP). As a result, the phosphorylated histone H2AX was decreased as DNA damage is repaired. We are still analyzing the status of some other histone modifications by using chromatin IP method. As for the chromatin status at the DNA damage site by I-Sce1 by using real-time PCR for the sensitivity for MNase, we observed the chromatin opening at the damage site. We will analyze the chromatin status after DNA repair in the near future.

Free Research Field

放射線生物学