Development of a reliable analytical method for endogenous hormones applicable to various animal species

2016 Fiscal Year Final Research Report

• Project/Area Number: 26550048
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Environmental impact assessment
• Research Institution: Ehime University
• Principal Investigator: Kunisue Tatsuya 愛媛大学, 沿岸環境科学研究センター, 教授 (90380287)
• Co-Investigator(Renkei-kenkyūsha): TANABE SHINSUKE 愛媛大学, 沿岸環境科学研究センター, 教授 (60116952)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 甲状腺ホルモン / 機器分析 / 遊離型 / タンパク結合型 / ステロイドホルモン

Outline of Final Research Achievements

The present study aimed to develop a sensitive and reliable analytical method for endogenous hormones, especially free thyroid hormones (THs) that play important role in the brain development, in serum/plasma of various animal species using ultrafiltration (UF) in combination with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), which can resolve the interferences encountered due to the nonspecific reactivity of anti-hormone antibodies used in immunoassay (IA) methods. UF at 1100 × g and 37°C for 30 minutes with a 30 kDa ultrafiltration device (Centrifree YM-30) yielded excellent precision. Acceptable accuracy (recovery: 100-110%) and intra- and inter-day precision (CV: <10% and <12%, respectively) were obtained, when triplicate analyses in three different days were conducted using bovine serum. The developed analytical method was successfully applied to the determination of free THs in plasma samples of humans and cats.

Free Research Field

環境分析化学