Ultra-high sensitive identification of unknown lipid-degrading microorganism in activated sludge to develop of microbiological control for membrane bioreactor

2015 Fiscal Year Final Research Report

• Project/Area Number: 26550105
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Design and evaluation of sustainable and environmental conscious system
• Research Institution: Kumamoto Industrial Research Institute
• Principal Investigator: Tanaka Ryoichi 熊本県産業技術センター(ものづくり室、材料・地域資源室、食品加工室), その他部局等, 研究主任 (50468063)
• Co-Investigator(Renkei-kenkyūsha): HORI Tomoyuki 産業技術総合研究所, 環境管理技術研究部門, 主任研究員 (20509533)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: Stable isotope probing / 16S rRNA gene sequencing / 長鎖脂肪酸分解 / Activated sludge / Membrane bioreactor

Outline of Final Research Achievements

We focused on the long chain fatty acid degradation that is one of rate-limiting step on lipid degrading process in the activated sludge treatment, unknown microorganism involved in degrading palmitic acid in the activated sludge was identified with a new fusion method of RNA-stable isotope probing (SIP) and 16S rRNA gene deep sequencing. Furthermore, rare species of microorganism taking in palmitic acid were revealed. The new powerful method of RNA-SIP and next generation sequencer technology was demonstrated to be useful in ultra-high sensitive identification of unknown microorganisms in environment.
To develop control technology of a high performance membrane bioreactor (MBR) for lipid-degradation, a small scale MBR was operated using wastewater including the olive oil, and microbes induced fouling and functional decline on the MBR in oily wastewater treatment were revealed.

Free Research Field

応用微生物学