Development of novel molecular imaging method for visualizing pluripotency of iPS cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 26560200
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: University of Tsukuba
• Principal Investigator: KANO Hideaki 筑波大学, 数理物質系, 准教授 (70334240)
• Co-Investigator(Kenkyū-buntansha): NISHMURA Ken 筑波大学, 医学医療系, 助教 (80500610)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: ラマン / CARS / iPS細胞

Outline of Final Research Achievements

Induced pluripotent stem cells (iPS cells) have potential to differentiate wide variety of tissues and organs. Recently, various kinds of studies have been carried out in order to apply iPS cells to tissue engineering and regenerative medicine. However, it is still difficult to find pluripotency of living iPS cells without staining or molecular tagging. In the present study, we have performed molecular vibrational imaging of iPS cells using coherent anti-Stokes Raman scattering (CARS) microscopy to explore the spectroscopic signatures of pluripotency. We used home-built multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopic system. As samples, we used different two kinds of iPS cell colony; 5- and 7-days ones after introducing four factor for the reprograming process. The intranuclear CARS spectral profiles in 5- and 7-days shows spectroscopic difference in two bands around 1560 and 790 cm-1. These might be useful to marker bands for pluripotency.

Free Research Field

分子分光学・分子分光イメージング