Evaluation of minor histocompatibility antigens by kidney transplant model derived from somatic cell cloned animals

2016 Fiscal Year Final Research Report

• Project/Area Number: 26560212
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: The University of Tokushima (2015-2016); Yamaguchi University (2014)
• Principal Investigator: Otoi Takeshige 徳島大学, 大学院生物資源産業学研究部, 教授 (30311814)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: クローン / 腎移植 / ミトコンドリア

Outline of Final Research Achievements

We tried to examine the effect of different mitochondrial DNA (mtDNA) by the transplantation of organ to donors that provided somatic cells using cloned animals. However, we could not obtain cloned cats for transplantation of organs to somatic cell donor cats.Thus, the effect of trichostatin A (TSA) on the development of interspecies cloned embryos (cat somatic cells / porcine or bovine enucleated oocytes) was investigated. TSA treatment had beneficial effects on the development of Cat-Cow cloned embryos but not of Cat-Pig cloned embryos. These results indicate that the difference in mitochondrial DNA may affect the development of cloned embryos.

Free Research Field

生殖工学