Isolation strategy of iPS cells that enable to maintain under xeno feeder free condition.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26560219
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: Taoka Masato 首都大学東京, 理工学研究科, 准教授 (60271160)
• Co-Investigator(Kenkyū-buntansha): IZUMI Tomonori 山口大学, 医学(系)研究科, 准教授 (00261694) ; SHIMAMOTO Akira 広島大学, 医歯薬保健学研究院, 准教授 (70432713)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: プロテオミクス / 糖鎖 / 細胞表面タンパク質

Outline of Final Research Achievements

Detailed knowledge of cell-surface proteins of iPS cells is necessary to isolate a well-defined population and enhance their translational potential. To identify and quantify proteins specific to membrane with high throughput, we developed a normal phase chromatographic approach with STAGE tip strategy. Through the approach, we identified over 1400 of cell-surface and N-linked glycoproteins from 500 micro g of proteins extracted from iPS cells cultured with xeno- and feeder-free condition. Compared them with the proteins on the surface of the original cells before obtaining pluripotency and embryoid body cells after differentiation by label-free proteomics quantitation, we obtained the 10 candidate proteins specific for iPS cells in xeno- and feeder-free condition.

Free Research Field

生化学