in vito reconstruction of 3-dimensional brain tissue using human cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26560247
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Tohoku Institute of Technology
• Principal Investigator: Suzuki Ikuro 東北工業大学, 大学院工学研究科, 准教授 (90516311)
• Research Collaborator: KIMURA Tsuyosi
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ヒトiPS細胞由来ニューロン / 3次元培養 / 脱細胞組織 / 神経細胞シート

Outline of Final Research Achievements

This study aimed to develop the 3-dimensional culture techniques using decellualized brain tissue and layered neuronal sheet in order to construct a biological brain tissue model. We confirmed that human iPSC-derived neuronal networks were cultured on the prepared decellularized pig brain tissue. We also found that a solution of decellularized brain tissue promote the survival and the elongation of neurites in cultured human iPS cell-derived neurons. These results suggest that the culture method using decellularized tissue are useful to 3-dimensional brain tissue model. Furthermore, we found that hiPSC-derived neuron sheet with maintain function can be detached and layered using temperature sensitive substrate.

Free Research Field

神経医工学