Gene-activating scaffolds for in vivo tissue regeneration

2016 Fiscal Year Final Research Report

• Project/Area Number: 26560250
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: OYANE Ayako 国立研究開発法人産業技術総合研究所, ナノ材料研究部門, 主任研究員 (50356672)
• Co-Investigator(Renkei-kenkyūsha): KIKUCHI Masanori 物質・材料研究機構, 機能性材料研究拠点, グループリーダー (00354267) ; WANG Xiupeng 産業技術総合研究所, 健康工学研究部門, 主任研究員 (70598789) ; ITO Atsuo 産業技術総合研究所, 健康工学研究部門, グループリーダー (30356480) ; YOSHINAKA Kiyoshi 産業技術総合研究所, 健康工学研究部門, 主任研究員 (90358341) ; TSURUSHIMA Hideo 筑波大学, 医学医療系, 准教授 (50315470)
• Research Collaborator: ARAKI Hiroko 産業技術総合研究所, ナノ材料研究部門, テクニカルスタッフ
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: DNA / リン酸カルシウム / 医療用輸液 / 遺伝子導入 / トランスフェクション / 細胞足場 / ナノコンポジット

Outline of Final Research Achievements

Calcium phosphate (CaP) layers immobilizing DNA (DNA-CaP composite layers) would be useful as surface components of tissue engineering scaffolds. This is because DNA-CaP composite layers possess a biocompatible surface suitable for cell growth and can control the cell behavior (differentiation, proliferation, etc.) through surface-mediated gene delivery. In this study, we fabricated DNA-CaP composite layers via coprecipitation in supersaturated CaP solutions that are prepared from infusion fluids. We shed light on the processes of coprecipitation and formation of the DNA-CaP composite layers in the supersaturated CaP solutions. Furthermore, we varied the coprecipitation conditions, i.e., coprecipitation time and Ca and P concentrations in solution, and obtained a guideline for the fabrication of DNA-CaP composite layers with high gene delivery capability and good cell compatibility.

Free Research Field

生体医工学, 生体材料学,