Analysis of p130Cas-mediated regulation of glucose metabolism

2015 Fiscal Year Final Research Report

• Project/Area Number: 26560410
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied health science
• Research Institution: Nadogaya Hospital (Laboratory for Mechanical Medicine, Nadogaya Research Institute)
• Principal Investigator: Sawada Yasuhiro 社会医療法人社団蛍水会名戸ヶ谷病院(名戸ヶ谷研究所メカノメディスン部門), 名戸ヶ谷研究所メカノメディスン部門, 部門長 (50313135)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: メカノトランスダクション

Outline of Final Research Achievements

We generated muscle-, endothelium-, and osteocyte-specific p130Cas knockout (KO) mice by crossing floxed-p130Cas mice with transgenic mice specifically expressing Cre-recombinase in those individual tissues. Since none of these conditional p130Cas KO mice was embryonic lethal, we could analyze their phenotypes at an adult age (10 weeks after birth). Osteocyte-specific p130Cas KO mice exhibited apparently altered bone metabolism. In endothelium-specific p130Cas KO mice, vascular network formation appeared abnormal. We are currently investigating the molecular mechanism behind those phenotypes with specific reference to responses to mechanical perturbations. In contrast, no obvious phenotype was observed in muscle-specific p130Cas KO mice, which showed no difference from their control mice at glucose tolerance test, either with normal diet or after 12-week high fat diet. However, we have noted a possible role of the interstitium in glycolipid metabolism.

Free Research Field

メカノバイオロジー