Exploring RNA modifications using enzymatic probe and its applications

2015 Fiscal Year Final Research Report

• Project/Area Number: 26560442
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Chemical biology
• Research Institution: The University of Tokyo
• Principal Investigator: Suzuki Takeo 東京大学, 工学(系)研究科(研究院), 講師 (90533125)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: RNA修飾 / 修飾酵素 / 特異的標識 / 網羅的探索

Outline of Final Research Achievements

We aimed to explore transcriptome-wide RNA modifications with new techniques using covalent complexes between RNA modification and RNA modifying enzyme. We transiently expressed a modifying enzyme of interest in HeLa cells that were cultured under supplying a nucleic acid analogue molecule and could collect bound RNA from immune-precipitated RNA-enzyme complex in the analogue molecule dependent manner. Moreover, we examined a novel RNA modification labeling method based on enzymatic conversion of specific RNA modifications followed by nucleophilic substitution reaction. The method may provide a new solution to detect those specific modifications.

Free Research Field

分子生物学