2017 Fiscal Year Final Research Report
Study of high throughput screening based on chemical imaging sensor
| Project/Area Number |
26620113
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | Saitama University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | 二次元電気化学センサ / LAAS / マイクロビーズ / アルコール脱水素酵素 / ホメピゾール / スクリーニング |
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| Outline of Final Research Achievements |
We proposed the screening system based on the Light Addressable Amperometric Sensor (LAAS) which enables high throughput screening for drag discovery. The system can image a distribution of enzyme reaction under an optical microscope. We measured enzyme activity of microbeads spread between a LAAS sensor and a gel electrolyte. The enzyme modified microbeads that treated with fomepizole (inhibitor) can be discriminated from untreated one. We confirmed that the system can evaluate the efficacy of the inhibitor. There is the possibility of the drag screening on biological cells because the size of the microbead close to a cell.
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| Free Research Field |
センサシステム
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