Analysis of dynamics of nucleic acid in cytosol

2015 Fiscal Year Final Research Report

• Project/Area Number: 26630421
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Nakamura Chikashi 東京農工大学, 工学(系)研究科(研究院), 客員教授 (40357661)
• Co-Investigator(Kenkyū-buntansha): IKEBUKURO Kazunori 東京農工大学, 大学院工学研究院, 教授 (70251494)
• Co-Investigator(Renkei-kenkyūsha): SUZUKI Makoto 東北大学, 大学院工学研究科, 教授 (60282109)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 分子クラウディング / ナノニードル / モレキュラービーコン / 核酸分子 / 結合速度定数

Outline of Final Research Achievements

In this study, we evaluated hybridization rate (association rate constant) of a molecular beacon (MB) with nucleic acids in molecular crowding condition mimicking intracellular environment. Furthermore, association rate constant of intracellular mRNA was directly analyzed by use of MB immobilized nanoneedle. In buffer solution simulated molecular crowding environment by addition of 20wt% PEG, association rate constant of s-oligo was higher than that of DNA. The constant of mRNA obtained by in vivo experiment exceeded all of the above-mentioned results. This result suggested that the diffusion coefficient of mRNA in cytosol is high compared to the previous reports.

Free Research Field

ナノ細胞工学