Novel production of therapeutic protein by using mammalian cell culture with the addition of chemical chaperones

2016 Fiscal Year Final Research Report

• Project/Area Number: 26630433
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Osaka University (2015-2016); The University of Tokushima (2014)
• Principal Investigator: Omasa Takeshi 大阪大学, 工学研究科, 教授 (00252586)
• Research Collaborator: ONITSUKA Masayoshi ; KIKAWADA Takahiro
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: バイオ医薬品 / ケミカルシャペロン / トレハロース / 抗体医薬 / CHO細胞 / 品質管理

Outline of Final Research Achievements

To investigate the mechanism of antibody aggregation during cultivation, the aggregation was analyzed during cultivation. The aggregation was observed in CHO cell. We have developed the CHO cell culture methods to suppress antibody aggregation by trehalose addition. Despite reducing aggregation, the cultivation method causes the inhibition of cell growth due to the increased osmotic pressure by trehalose addition. By introduction of the trehalose transporter we can attain both suppressed antibody aggregation and improved cell growth.

Free Research Field

生物化学工学