Development of new amyloid beta-GFP fusion protein which is a useful tool for the analysis of the synaptic function of amyloid beta monomers and oligomers

2015 Fiscal Year Final Research Report

• Project/Area Number: 26640023
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: OCHIISHI TOMOYO 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (30356729)
• Co-Investigator(Kenkyū-buntansha): KAKU Masami 茨城県立医療大学, 保健医療学部, 嘱託助手 (30646261)
• Co-Investigator(Renkei-kenkyūsha): DOI Motomichi 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (50344213) ; EBIHARA Tatsuhiko 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (00344119)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: アルツハイマー病 / アミロイドβタンパク質 / シナプス / オリゴマー / トランスジェニックマウス

Outline of Final Research Achievements

The intracellular accumulation of Amyloid-β (Aβ) oligomers critically contributes to disease progression in Alzheimer’s disease (AD) and can be contribute to the potential target of AD therapy. We developed Aβ-GFP fusion proteins that are oligomerized and visualize their dynamics inside cells. We also developed the Aβ-GFP transgenic mice that express Aβ-GFP fusion protein in neurons. The behavior analysis of this transgenic mice presented impaired retention memory compared with the non-transgenic mice in young age. These results suggest that the Aβ-GFP transgenic mouse is useful tool to investigate the toxicity of Aβ oligomer and pathogenic mechanisms of memory deficit of AD.

Free Research Field

神経細胞生物学