2016 Fiscal Year Final Research Report
Development of the next generation optogenetics tools by projection specific labeling using multiple selection
| Project/Area Number |
26640041
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | アデノ随伴ウイルスベクター / Creリコンビナーゼ / 経シナプス逆行性 |
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| Outline of Final Research Achievements |
Adeno-associated virus (AAV) which express retrogradely transsynaptic Cre recombinase, Cre-TTC (Cre recombinase fused with tetanus toxin C terminal fragment), was infected targeted brain area. Then, AAV which express fluorescent protein in the presence of Cre recombinase was infected. To test this, dopaminergic neurons in the Substance Niger were applied. Neurons in the striatum was infected AAV-CMV-Cre-TTC-2A-mCherry. Neurons in the substantia niger was injected AAV-CMV-FLEX-GFP. As a result, GFP fluorescent was observed in the neurons in the substantia niger suggested that Cre-TTC was retrogradely transsynaputically transported and worked in the neurons.
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| Free Research Field |
神経科学
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