2014 Fiscal Year Final Research Report
Analysis of target genes of the sex-determining gene, Sry
| Project/Area Number |
26640055
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | The University of Tokushima |
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Principal Investigator |
TACHIBANA Makoto 徳島大学, 疾患酵素学研究センター, 教授 (80303915)
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| Project Period (FY) |
2014-04-01 – 2015-03-31
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| Keywords | 性決定遺伝子 |
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| Outline of Final Research Achievements |
In XY mammal, Sex-determining region Y (Sry) transiently expresses in undifferentiated embryonic gonads, thereby inducing testis differentiation. I tried to establish antibodies that recognize endogenous mouse SRY protein to identify SRY target genes. Gonads from mouse embryos at embryonic day 11.5 were subjected to ChIP analysis with the anti-SRY antibodies. Next, ChIPed DNA was applied to the next generation sequencing analysis to identify candidates of SRY target genes.
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| Free Research Field |
分子生物学
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