Random but systematic establishment of the cell lineage-ablated mouse lines and their rescue

2016 Fiscal Year Final Research Report

• Project/Area Number: 26640058
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: Ishida Yasumasa 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (10221756)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 遺伝子トラップ / ＵＰＡＴｒａｐ / ＤＴｒａｐ / ジフテリア毒素 / 細胞系譜の除去 / ＥＳ細胞 / マウス

Outline of Final Research Achievements

When we perform gene-trapping experiments with mouse undifferentiated embryonic-stem (ES) cells by using a novel promoter-trapping strategy termed DTrap, in which the diphtheria-toxin (DT) gene is involved as a negative selectable marker, we are able to establish only the ES-cell clones whose transcriptionally silent genes had been trapped (disrupted). In the current research, we have tried to create the cell lineage-ablated mouse lines by performing complementation of mouse tetraploid embryos with such 'DTrapped' ES cells. We also aimed at rescuing the complemented tetraploid embryos by co-injecting the second type of wild-type or mutant ES cells.

Free Research Field

マウスの分子遺伝学と免疫学