Development of rapid system for generation of consomic mice via chromosome transfer and elimination technologies

2016 Fiscal Year Final Research Report

• Project/Area Number: 26640059
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: Tottori University
• Principal Investigator: KAZUKI Yasuhiro 鳥取大学, 医学(系)研究科(研究院), 准教授 (90403401)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: リサーチバイオリソース

Outline of Final Research Achievements

Use of consomic mice played an important role to identify the responsible gene of the multiple factor disease. However, it was a problem that several years and a lot of labor for the manufacture process have been needed. In this study, I aimed to generate rapid system for generation of consomic mice by constructing the consomic cells in vitro via chromosome transfer and elimination technologies. Using whole cell fusion and chromosome transfer technique, I succeeded in introducing a B6-deribed chromosome 11 into 129-deribed mouse ES cells in which the 129-deribed chromosome 11 can be eliminated. The system will be expected to be a useful tool for the generation of consomic mice rapidly.

Free Research Field

染色体工学