Establishment of system for direct detection of exosome

2015 Fiscal Year Final Research Report

• Project/Area Number: 26640091
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnostics
• Research Institution: Hiroshima University
• Principal Investigator: Tahara Hidetoshi 広島大学, 医歯薬保健学研究院(薬), 教授 (00271065)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 細胞外小胞 / エクソソーム

Outline of Final Research Achievements

Exosome is extracellular nano-vesicles secreted from different type of cells. However, there is no significant flow-based instrument to detect exosome because of high background noise under 500nm size detection by current flow cytometers. To overcome this problem, we used a spectral cell analyzer, SP6800 (Sony corporation) which adopts a new method to measure the full spectrum of fluorescence with a high degree of accuracy. We purified exosome and stained with Cellview reagent, and significant and specific population is detected very small size population, which is distinguished from noise population. We also found that Alexa488 labeled CD9 and Alexa647 labeled CD63 were presented in purified exosomes, but exosomes are heterogeneous population. We also tried to detect cancer specific exosome derived from pancreatic cancer, and the spectral cell analyzer SP6800 can be used to detect exosome particle directly.

Free Research Field

細胞分子生物