2016 Fiscal Year Final Research Report
Study of stress-related disorder monitored by plasma proteomics
| Project/Area Number |
26640115
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 血漿 / プロテオミクス / 質量分析 / ストレス障害 / モデルマウス / 血液 |
|---|
| Outline of Final Research Achievements |
Human plasma is by far the most commonly sampled diagnostic biospecimen and a potentially informative resource of extreme importance for characterizing proteomes. However, the existence of high-abundance proteins and the large heterogeneity of protein modification and fragmentation make precise analysis of proteins challenging. In this study, we developed the novel pretreatment method of plasma, which we call PF method, for preseparating plasma proteins. Based on this method, we achieved high-quality comparative analysis of plasma proteins and accurately detected alteration of plasma proteome profiling correlating with stress-related disorder.
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| Free Research Field |
プロテオミクス
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