Basic technology development for Shark nanobody using antigen protein produced by wheat cell-free system

2015 Fiscal Year Final Research Report

• Project/Area Number: 26640130
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: System genome science
• Research Institution: Ehime University
• Principal Investigator: SAWASAKI TATSUYA 愛媛大学, プロテオサイエンスセンター, 教授 (50314969)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: ナノボディ / 無細胞 / サメ / 抗体 / ドチザメ / 免疫

Outline of Final Research Achievements

We were successful to make an environment for the immunological experimental using three kinds of banded dogfishes that are harvested from off the coast of Matsuyama, Ehime Prefecture, calling Iyonada. Furthermore, we established good methods for handling, feeding, blood sampling and labeling of these sharks. Next we isolated a pure total RNA from bloods of these sharks and it is used for analysis of whole transcriptome by RNA seq. These results showed sequences of IgNAR coding shark nanobody.

Free Research Field

蛋白質科学