Basic study of preservation technologies using normal temperature vitrification

2016 Fiscal Year Final Research Report

• Project/Area Number: 26640140
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Conservation of biological resources
• Research Institution: Mukogawa Women's University Junior College Division
• Principal Investigator: YOSHIDA Toru 武庫川女子大学短期大学部, 食生活学科, 教授 (00378952)
• Co-Investigator(Renkei-kenkyūsha): SAKURAI Minoru 東京工業大学, バイオ研究基盤支援総合センター, 教授 (50162342)
• Research Collaborator: SAKAGAMI Aya ; YANO Megumu ; Yokoro Miyuki
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ガラス化 / 常温保存 / トレハロース / LEAペプチド / 極限環境生物 / アルテミア / クリプトビオシス

Outline of Final Research Achievements

In this study, we focused on the distinctive features of "cryptobiosis" preserved among extremophiles, such as Artemia franciscana, an aquatic arthropod, and investigated the optimal conditions for normal temperature vitrification using gelatinous models. We reported the causal relationship between the amount of water content and the degree of vitirification after adding various kinds of saccharides to agarose-based gelatinous models. We also confirmed that normal temperature vitrification depends on the concentration of LEA (Late Embryogenesis Abundant) peptide and nonreducing disaccharide, trehalose using a quail egg model. We concluded that the precise ratio of LEA peptide to trehalose is important in determining the success of normal temperature vitrification for the preservation of bioresource materials.

Free Research Field

生物資源保存