2015 Fiscal Year Final Research Report
Anaerobic crystallization for protein crystallization
| Project/Area Number |
26650026
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | High Energy Accelerator Research Organization |
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Principal Investigator |
SENDA Miki 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 特任助教 (10707467)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | タンパク質の結晶化 / 嫌気条件下での結晶化 / 嫌気チャンバー / 結晶化の再現性向上 |
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| Outline of Final Research Achievements |
It’s essential to obtain crystals with high reproducibility for an X-ray protein crystallography. Anaerobic crystallization is useful to improve reproducibility of crystallization, because oxidation of protein solutions hampered the crystal formation. We’ve established typical procedures for the anaerobic crystallization and the anaerobic crystallization was tested for several proteins. Crystals were obtained with higher reproducibility under anaerobic condition than under aerobic condition. We believe that anaerobic crystallization is effective to crystallize a various kinds of proteins.
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| Free Research Field |
Protein crystallography
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