Elucidation of the molecular mechanism of the pressure dependent fluorescence change of the YFP mutants toward the development of a pressure sensor.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26650036
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Osaka University
• Principal Investigator: Imada Katsumi 大阪大学, 理学(系)研究科(研究院), 教授 (40346143)
• Co-Investigator(Renkei-kenkyūsha): WATANABE Nobuhisa 名古屋大学, シンクロトロン光研究センター, 教授 (70212321)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 蛍光タンパク質 / 結晶構造 / 圧力 / メカノバイオロジー

Outline of Final Research Achievements

Glycine insertion in β7 of YFP dramatically changes its fluorescence property dependent on hydrostatic pressure. In this study, we determined the crystal structures of the mutants and measured their fluorescence spectra under high pressure. We found the flipping of the carbonyl oxygen of the chromophore with the conformationl changes of the residues surrounding the chromophore under high pressure. These conformations are similar to those of YFP at normal pressure, suggesting these conformational changes cause the pressure response on the fluorescence property.

Free Research Field

生物物理学