Singe molecule force measurement of nucleic acid bidning proteins for understanding autism

2016 Fiscal Year Final Research Report

• Project/Area Number: 26650056
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Tanaka Motomasa 国立研究開発法人理化学研究所, 脳科学総合研究センター, チームリーダー (40321781)
• Research Collaborator: Komi Yusuke 国立研究開発法人理化学研究所, 脳科学総合研究センター, 研究員 (20565999) ; Shida Toshinobu 東京工業大学, 大学院・生命理工学研究科, 大学院生
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 一分子 / タンパク質構造 / 力学計測

Outline of Final Research Achievements

Protein dynamically fluctuates in solution and adopts various distinct conformations. However, the measurement of such conformational heterogeneity of protein is technically difficult and the determination of a conformational space of protein is challenging. So far, single molecule force measurement with optical tweezers is a promising technique to address it, but it is not easily applied to nucleic acid proteins because the single protein molecule is tethered by a DNA handle linker which may alter a conformational space of the captured nucleic acid protein. In this study we developed a novel method of single molecule force measurement, using polyethylene glycol as a handle for single molecule of nucleic acid proteins.

Free Research Field

構造生物学