Raft-like structures in the ER: Implication of apoptosis, autophagy, and lipid droplet formation

2015 Fiscal Year Final Research Report

• Project/Area Number: 26650066
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: TAGAYA MITSUO 東京薬科大学, 生命科学部, 教授 (30179569)
• Co-Investigator(Kenkyū-buntansha): TANI Katsuko 東京薬科大学, 生命科学部, 教授 (40266896)
• Co-Investigator(Renkei-kenkyūsha): WAKANA Yuichi 東京薬科大学, 生命科学部, 助教 (90635187)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 小胞体 / ラフト / syntaxin17 / オートファジー / 脂肪滴 / VAP-A/B

Outline of Final Research Achievements

The MAM（mitochondria-associated membrane）and some of the other ER subdomains appear to have raft-like structures. In the present project we made the following findings about raft-localized proteins, syntaxin 17 (Syn17) and VAP-A/B: (1) Syn17 regulates apoptosis. (2) Upon nutrient deprivation, Syn17 redistributes from the raft-like structure (MAM) to the non-raft-like structure (autophagosomes).(3) Syn17 in cooperation with another raft-localized protein, SNAP23, regulates lipid droplet formation. (4) VAP-A/B localize to the ER-Golgi contact site and regulate the raft-like structure of the trans-Golgi network.

Free Research Field

細胞生物学