Development of cell culture substratum that restrict morphology of cell aggregate during 3D-morphogenesis

2015 Fiscal Year Final Research Report

• Project/Area Number: 26650071
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Yonemura Shigenobu 国立研究開発法人理化学研究所, ライフサイエンス技術基盤研究センター, チームリーダー (60192811)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 上皮細胞 / 細胞接着 / 形態形成 / 微細加工

Outline of Final Research Achievements

To restrict the initial morphology of epithelial cell aggregates is the purpose of this study. I designed a metal mold to make PDMS which has V-shaped grooves. The initial morphology of epithelial cell aggregates was successfully restricted by seeding cell onto this PDMS. By tuning the depth or width of V-shape grooves, observation of several number of V-shaped grooves was possible. As a result, during cell adhesion, the aspect ratios of the cell aggregates became low quickly in a special time window, indicating that epithelial cells have a mechanism to correct their aggregate shape to ball during their adhesion.

Free Research Field

細胞生物学