Development of model system to understand nematode infection mechanisms.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26650102
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kumamoto University
• Principal Investigator: Sawa Shinichiro 熊本大学, 大学院先端科学研究部(理), 教授 (00315748)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 植物感染性線虫 / 感染機構 / M. incognita / A. thaliana

Outline of Final Research Achievements

Developmental plasticity is one of the most striking features of plant morphogenesis, as plants are able to vary their shapes in response to environmental cues. Root-knot nematodes (RKNs) are known to parasitize multiple species of rooting plants and to induce characteristic tissue expansion called galls or root-knots on the roots of their hosts by perturbing the plant cellular machinery.
In this study, we deciphered the molecular mechanism of gall formation with an in vitro infection assay system using RKN Meloidogyne incognita, and the model plant Arabidopsis thaliana. By taking advantages of this system, we performed next-generation sequencing (NGS)-based transcriptome profiling to know molecular mechanisms of nematode infection mechanisms, and found many categories of gene signaling are responsible for nematode infection steps.

Free Research Field

植物分子遺伝発生学