Transcriptome analysis using an isolated nephron segment to identify important factors for euryhalinity of fish

2015 Fiscal Year Final Research Report

• Project/Area Number: 26650110
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Morphology/Structure
• Research Institution: The University of Tokyo
• Principal Investigator: HYODO SUSUMU 東京大学, 大気海洋研究所, 准教授 (40222244)
• Co-Investigator(Kenkyū-buntansha): KURAKU SHIGEHIRO 国立研究開発法人理化学研究所, ライフサイエンス技術基盤研究センター, ユニットリーダー (40391940)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 広塩性 / オオメジロザメ / 腎臓 / ネフロン / 膜輸送体 / ホルモン / プロラクチン

Outline of Final Research Achievements

The aim of this study is to identify crucial factors that contribute to the euryhalinity of fish by using bull shark (Carcharhinus leucas), a unique euryhaline species in cartilaginous fishes, as a model organism. We conducted RNA-seq of bull shark kidney using seawater- and freshwater-acclimated fish, and have found a large number of important genes; expression levels of which were considerably up-regulated or down-regulated following the freshwater acclimation. The late distal tubule and the collecting tubule of the bull shark nephron are considered to be crucial segments for the euryhalinity of bull shark. We successfully found candidate factors important for the euryhalinity, such as prolactin, insulin-like growth factors, and stanniocarcin.

Free Research Field

魚類生理学、比較生理学、比較内分泌学