2016 Fiscal Year Final Research Report
The molecular mechanism of uniparental inheraitance of mtDNA analyzed by combining the optical tweezers and microfluidic device.
| Project/Area Number |
26650111
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphology/Structure
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 光ピンセット / マイクロ流路 / ミトコンドリア / 片親遺伝 |
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| Outline of Final Research Achievements |
Living organisms consist of diverse tissues, cells and organelles, which can be compared to a complex society with various groups and individuals. To decipher the characteristics of individual cells and connections between them, it would be necessary to establish technologies to isolate individual cells for further molecular analyses. For this, we aimed to combine the optical tweezers and microfluidic devises. With this technique, the molecular mechanism of uniparental inheritance of mtDNA was analyzed using Cryptococcus neoformans as a model system, which revealed the active elimination of α-mtDNA in the early developing zygotes that lead to the uniparental inheritance of mtDNA.
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| Free Research Field |
植物分子遺伝学
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