2016 Fiscal Year Final Research Report
Is actin indispensable for cell survival? A study with Chlamydomonas
| Project/Area Number |
26650136
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Evolutionary biology
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| Research Institution | Chuo University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KAMIYA Ritsu 学習院大学, 理学部, 客員教授 (10124314)
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| Research Collaborator |
UEKI Noriko 中央大学, 理工学部, 共同研究員
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | アクチン / 重合 / amiRNA / 細胞分裂 / 繊毛形成 |
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| Outline of Final Research Achievements |
Is the cytoskeletal protein actin indispensable for eukaryotic life? In this study, we asked this fundamental question using mutants of a unicellular green alga, Chlamydomonas, which has only two actin genes coding for two discrete types of actins, CrA and NAP. We previously isolated a null mutant of CrA. Here, we examined the effect of expression reduction of NAP using amiRNA in the CrA-less mutant. A transformed strain that expressed no CrA and ~10% of NAP was viable, although another strain that expressed no CrA and no detectable NAP displayed abnormal growth. These results suggest that actin is indispensable, but only a small amount of it is enough for cells to survive.
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| Free Research Field |
細胞生物学
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