内蔵脂肪症候群による遺伝子不安定性の解析

2014 Fiscal Year Research-status Report

• Project/Area Number: 26650174
• Research Institution: Nagasaki University
• Principal Investigator: 草野 洋介 長崎大学, 医歯薬学総合研究科(医学系), 研究員 (70325637)
• Co-Investigator(Kenkyū-buntansha): 有馬 和彦 長崎大学, 医歯薬学総合研究科(医学系), 講師 (30423635) ; 西村 貴孝 長崎大学, 医歯薬学総合研究科(医学系), 助教 (80713148) ; 安部 恵代 長崎大学, 医歯薬学総合研究科(医学系), 准教授 (90372771)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: ダイエット

Outline of Annual Research Achievements

「内蔵脂肪症候群による遺伝子不安定性の存在」を明らかにする事を最終目的に二つの検討を行う。第一の検討では内蔵脂肪症候群と健常群の遺伝子不安定性を比較する。第二の検討では内蔵脂肪症候群を有する参加者における正常血糖状態と高血糖状態での遺伝子不安定性を司る遺伝子群の発現量を比較する。本研究により、生理的な内蔵脂肪の量的および質的多型性への理解が深まるだけでなく、内蔵脂肪症候群が遺伝子不安定性を介して、広く遺伝子関連病態へ影響を与えている事を説明する新しい基礎的知見を得る事が出来る。対象者と説明された同意。対象はダイエット外来を受診し、研究に同意の得られた肥満状態の受診者。同意は過不足なく説明された上で書面にて研究への協力を確認する。
生体資料の採取と遺伝子の精製と厳重な保管がおこなわれた。　研究参加への意思が確認された対象者は経口糖負荷試験等の採血を必要とする診療行為の際に新たな侵襲を伴わずに血液が採取される。血清試料：血清試料用血液検体は血液凝固促進剤による血液凝固が完全に完了する、最低でも室温３０分間経過後に遠心分離によって血清が上清として分離された。得られた血清試料は速やかに深低温下に保管される。血漿試料：血漿試料用血液検体は抗血液凝固剤の存在下で成功的な転倒混和が行われた後に可及的速やかに遠心分離によって血漿が上清として分離される。得られた血漿試料は速やかに深低温化に保管される遺伝子試料：遺伝子試料用血液検体からは末梢血単核球細胞の崩壊を回避する保存環境下で自動遺伝子分離精製機械を用いて純度の高い遺伝子が分離された。可及的速やかに個人が容易に特定できない様に匿名化された。精製後の遺伝子は遺伝子分解酵素の働きを阻害する1mM EDTA含有10mMトリス緩衝溶液を用いて適正な酸塩基状態に保たれた溶媒に再融解された状態で深低温下に保管された。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

おおむね計画通りに進行している

Strategy for Future Research Activity

研究計画に沿って研究を進める。

Causes of Carryover

研究同意者の参加人数により小額の次年度使用消耗品費が発生した。

Expenditure Plan for Carryover Budget

研究計画の通りに遂行する。

Research Products

• [Journal Article] Association between the hemoglobin levels and hypertension in relation to the BMI status in a rural Japanese population: the Nagasaki Islands Study. (2015)
  • Author(s): Shimizu Y, Nakazato M, Sekita T, Kadota K, Arima K, Yamasaki H, Takamura N, Aoyagi K, Maeda T.
  • Journal Title: Intern Med. Volume: 53 Pages: 1-2
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Association between hemoglobin A1c and carotid atherosclerosis in rural community-dwelling elderly Japanese men. (2015)
  • Author(s): Shimizu Y, Nakazato M, Sato S, Koyamatsu J, Yamanashi H, Nagayoshi M, Kadota K, Hayashida N, Yamasaki H, Kusano Y, Takamura N, Aoyagi K, Maeda T.
  • Journal Title: J Physiol Anthropol. Volume: 34 Pages: 16-17
  • DOI: 10.1186/s40101-015-0054-6.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Risk factors of adverse events during treatment in elderly patients with rheumatoid arthritis: an observational study. (2015)
  • Author(s): Iwanaga N, Arima K, Terada K, Ueki Y, Horai Y, Suzuki T, Nakashima Y, Kawashiri SY, Ichinose K, Tamai M, Nakamura H, Aoyagi K, Kawakami A, Origuchi T.
  • Journal Title: Int J Rheum Dis. Volume: 1 Pages: 1-2
  • DOI: 10.1111/1756-185X.12348
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Upregulation of Thrombospondin 1 Expression in Synovial Tissues and Plasma of Rheumatoid Arthritis: Role of Transforming Growth Factor-β1 toward Fibroblast-like Synovial Cells. (2015)
  • Author(s): Suzuki T, Iwamoto N, Yamasaki S, Nishino A, Nakashima Y, Horai Y, Kawashiri SY, Ichinose K, Arima K, Tamai M, Nakamura H, Origuchi T, Miyamoto C, Osaki M, Ohyama K, Kuroda N, Kawakami A.
  • Journal Title: J Rheumatol. Volume: 1 Pages: 1-2
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Successful treatment of Schnitzler syndrome with cyclosporine. (2014)
  • Author(s): Nakajima K, Takahashi M, Yamamoto M, Takahashi A, Sano S, Kodama H, Arima K, Ida H.
  • Journal Title: Int J Dermatol. Volume: 53 Pages: e361-363
  • DOI: 10.1111/ijd.12300
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Accuracy of spinal curvature assessed by a computer-assisted device and anthropometric indicators in discriminating vertebral fractures among individuals with back pain. (2014)
  • Author(s): Mizukami S, Abe Y, Tsujimoto R, Arima K, Kanagae M, Chiba G, Aoyagi K.
  • Journal Title: Osteoporos Int. Volume: 25 Pages: 1727-1734
  • DOI: 10.1007/s00198-014-2680-y
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Synovial inflammation assessed by ultrasonography correlates with MRI-proven osteitis in patients with rheumatoid arthritis. (2014)
  • Author(s): Kawashiri SY, Suzuki T, Nakashima Y, Horai Y, Okada A, Nishino A, Iwamoto N, Ichinose K, Arima K, Tamai M, Nakamura H, Origuchi T, Uetani M, Aoyagi K, Eguchi K, Kawakami A.
  • Journal Title: Rheumatology (Oxford) Volume: 53 Pages: 1452-1456
  • DOI: 10.1093/rheumatology/keu034
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Associations between renal impairment and anemia in older, rural Japanese men: the Nagasaki Island study. (2014)
  • Author(s): Shimizu Y, Sato S, Koyamatsu J, Yamanashi H, Nagayoshi M, Kadota K, Tamai M, Arima K, Yamasaki H, Kusano Y, Takamura N, Maeda T.
  • Journal Title: J Physiol Anthropol. Volume: 33 Pages: 7-8
  • DOI: 10.1186/1880-6805-33-7
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Height and drinking status in relation to risk of anemia in rural adult healthy Japanese men: the Nagasaki Islands study. (2014)
  • Author(s): Shimizu Y, Nakazato M, Sekita T, Kadota K, Miura Y, Arima K, Yamasaki H, Goto H, Takamura N, Aoyagi K, Maeda T.
  • Journal Title: Aging Male. Volume: 23 Pages: 1-6
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Combination of MRI-detected bone marrow oedema with 2010 rheumatoid arthritis classification criteria improves the diagnostic probability of early rheumatoid arthritis. (2014)
  • Author(s): Tamai M, Kita J, Nakashima Y, Suzuki T, Horai Y, Okada A, Koga T, Kawashiri SY, Iwamoto N, Ichinose K, Arima K, Yamasaki S, Nakamura H, Origuchi T, Uetani M, Fukushima A, Aoyagi K, Eguchi K, Kawakami A.
  • Journal Title: Ann Rheum Dis. Volume: 73 Pages: 2219-2220
  • DOI: 10.1136/annrheumdis-2013-205074
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Mental health problems and influencing factors in Japanese women 4 months after delivery. (2014)
  • Author(s): Yamamoto N, Abe Y, Arima K, Nishimura T, Akahoshi E, Oishi K, Aoyagi K.
  • Journal Title: J Physiol Anthropol. Volume: 33 Pages: 32-33
  • DOI: 10.1186/1880-6805-33-32
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Direct Infection of Primary Salivary Gland Epithelial Cells by Human T Lymphotropic Virus Type I in Patients With Sjögren's Syndrome. (2014)
  • Author(s): Nakamura H, Takahashi Y, Yamamoto-Fukuda T, Horai Y, Nakashima Y, Arima K, Nakamura T, Koji T, Kawakami A.
  • Journal Title: Arthritis Rheumatol. Volume: 67 Pages: 1096-1106
  • DOI: 10.1002/art.39009
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Epidemiology of Kienböck's disease in middle-aged and elderly Japanese women. (2014)
  • Author(s): Tsujimoto R, Maeda J, Abe Y, Arima K, Tomita M, Koseki H, Kaida E, Aoyagi K, Osaki M.
  • Journal Title: Orthopedics. Volume: 38 Pages: e14-18
  • DOI: 10.3928/01477447-20150105-54
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Distinguishing the cerebrospinal fluid cytokine profile in neuropsychiatric systemic lupus erythematosus from other autoimmune neurological diseases. (2014)
  • Author(s): chinose K, Arima K, Ushigusa T, Nishino A, Nakashima Y, Suzuki T, Horai Y, Nakajima H, Kawashiri SY, Iwamoto N, Tamai M, Nakamura H, Origuchi T, Motomura M, Kawakami A.
  • Journal Title: Clin Immunol. Volume: 157 Pages: 114-120
  • DOI: 10.1016/j.clim.2015.01.010
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] LONGITUDINAL ASSOCIATIONS BETWEEN PAINFUL KNEE OSTEOARTHRITIS AND DIFFICULTIES IN PHYSICAL FUNCTIONING AMONG COMMUNITY-DWELLING WOMEN (2015)
  • Author(s): K. Arima, T. Nishimura, Y. Abe, K. Aoyagi
  • Organizer: EULAR2015
  • Place of Presentation: Italy
  • Year and Date: 2015-06-13 – 2015-06-15
• [Remarks] 長崎大学大学院医歯薬学総合研究科公衆衛生学分野
  • URL: http://www.med.nagasaki-u.ac.jp/public-h/